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Research Facilities

Most departmental laboratories and associated instrumentation are located in the Medical Sciences I complex and adjacent Irvine Hall.

The labs contain a wide variety of equipment for research in molecular and cellular physiology and molecular biophysics. Knowledgeable users can help investigators in the department use this equipment for their projects.

Microscopy: fluorescence, ratiometric calcium imaging, confocal. (Cahalan, Hall labs)

Electrophysiology and cell microinjection: patch clamp, microelectrode recording (oocyte) setups. (Cahalan, Hall labs)

Molecular biology: protein (HPLC, FRLC) and nucleic acid work (electrophoresis, DNA sequencing, PCR, electroporation, gel photography); programs for analyzing protein and DNA sequences and for molecular visualization. (most labs)

Mass spectrometry: Orbitrap Fusion™ Lumos™ and LTQ-Orbitrap XL™ mass spectrometers (Thermo Fisher Scientific) for protein and peptide analysis by liquid chromatography-mass spectrometric analysis (LC-MS/MS, LC-MSn). Capabilities include detailed characterization of post-translational modifications, quantitative proteomics, whole-proteome profiling, protein interaction/structural analysis using cross-linking mass spectrometry (XL-MS) and intact protein analysis. (Huang lab)

Optical spectroscopy: Absorbance, fluorescence, step-scan FTIR, and circular dichroism. (Longmuir, White labs)

Physicochemical characterization: Differential scanning calorimetry, isothermal titration calorimetry, static and dynamic light scattering, BiaCore surface plasmon resonance. (Longmuir, White labs)

Library: The Department maintains the previous three to five years of major journals in physiology, biophysics, biochemistry, molecular biology, immunology, and cell biology, as well as journals of broad scientific interest, and reference books. Computers provide free access to on-line publications.

UC Irvine Facilities

  • Optical Biology Core Resource ›
  • BioImaging Interest Group ›
  • DNA Core Facility ›
  • DNA Array Core Facility ›
  • Transgenic Mouse Facility ›